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glmS [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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glmS [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

glutamine-fructose-6-phosphate transaminase
locus
BSU01780
pI
4.00
mw
65.00 kDa
function
cell wall synthesis
product
glutamine-fructose-6-phosphate transaminase
essential
yes
ec
2.6.1.16
synonyms
gcaA, ybxD

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|glmS ribozyme]
  • Gene

    Coordinates on the chromosome (coding sequence)
    200,277 -> 202,079

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • L-glutamine D-fructose 6-phosphate = L-glutamate D-glucosamine 6-phosphate (according to Swiss-Prot)
  • Structure

  • [PDB|2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) [Pubmed|18295797]
  • the ribozyme: [PDB|3G8S], [PDB|3G9C], [PDB|3G8T], [PDB|3G95], [PDB|3G96] (all for the ribozyme from ''Bacillus anthracis''), [PDB|2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • :
  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]
  • Expression and Regulation

    Operon

  • ''[protein|search|cdaA]-[protein|search|cdaR]-[protein|search|glmM]-[protein|search|glmS]'' [Pubmed|23192352]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|22211522]
  • Regulation

  • repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine [Pubmed|14343123]
  • ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([protein|search|glmS] [SW|ribozyme])
  • Regulatory mechanism

  • ''glmS'' [SW|ribozyme]: glucosamine 6-phosphate binds the leader mRNA, and a [SW|riboswitch] with [SW|ribozyme] activity cleaves off the ''[protein|search|glmS]'' section from the mRNA, resulting in stopp of transcript elongation
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]
  • A [protein|search|ncRNA] is predicted between '[protein|search|glmM]' and '[protein|search|glmS]' [PubMed|20525796]
  • Labs working on this gene/protein

  • [SW|Wade Winkler], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]
  • References

    Reviews

  • 18279655,24769284,12859215,20709015
  • The ''glmS'' Ribozyme

  • 18079181,16484375,16784238,15096624,16990543,17114942,16484375,15029187,17283212,16298301,19228039,21317896,21395279,24318896
  • Other Original Publications

  • 14343123,17981983,11160890,18295797,20525796,23192352,23876412,22211522