ribE

riboflavin synthase (alpha subunit)

Locus: BSU_23270

Isoelectric point: 5.84

Molecular weight: 23.33 kDa

Protein length: 215 aa

Gene length: 648 bp

Function: riboflavin biosynthesis

Product: riboflavin synthase (alpha subunit)

Essential: no

E.C.: 2.5.1.9

Synonyms: ribB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of riboflavin/ FAD

Gene

Coordinates: 2,429,600 2,430,247

The protein

Catalyzed reaction/ biological activity

• 2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H⁺ --> 5-amino-6-(D-ribitylamino)uracil + riboflavin (according to UniProt)

Structure

• 1I8D (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed

Expression and Regulation

Operons

• Genes: ribD-ribE-ribA-ribH-ribT
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • FMN-box: transcription termination, via FMN-box in the presence of FMN or FMNH₂, this is counter-acted upon binding of RibR, in FMN-box

  Regulation

  • expressed in the absence of FMN (FMN-box) PubMed
  • binding of FMN to the FMN-box RNA is facilitated by NusG-mediated transcription pausing PubMed
  • the FMN-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• GP203 (erm), available in Jörg Stülke's lab
• BKE23270 (ribE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT
• BKK23270 (ribE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT

References

Reviews

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Original publications

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