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mraY [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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mraY [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate), catalyzes the first commited membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I
locus
BSU15190
pI
8.00
mw
35.00 kDa
function
peptidoglycan precursor biosynthesis
product
phospho-N-acetylmuramoyl-pentapeptide-transferase
(meso-2,6-diaminopimelate)
essential
yes
ec
2.7.8.13
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes], [SW|membrane proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,587,926 -> 1,588,900

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) undecaprenyl phosphate = UMP Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)
  • Protein family

  • MraY subfamily (according to Swiss-Prot)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • forms banded patterns along the length of the cell, this pattern formation depends on [SW|MreB] [Pubmed|20233306]
  • Expression and Regulation

    Operon

  • ''[protein|search|murE]-[protein|search|mraY]-[protein|search|murD]-[SW|spoVE]-[protein|search|murG]-[protein|search|murB]-[SW|divIB]-[protein|search|ylxW]-[protein|search|ylxX]-[protein|search|sbp]'' [Pubmed|1391053]
  • Regulation

  • expressed during vegatative growth [Pubmed|1391053]
  • Biological materials

    FLAG-tag construct

  • GP2001 ''mraY-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab
  • References

  • 15131133,8436954,18791230,18081839,7734839,21937437,18672909,20537545,10790316,1391053,24895118,25127465,26620564,20233306,27226570,27312048