mrfA

ATP-dependent helicase, part of a novel nucleotide excision repair pathway

locus: BSU_22220

pI: 6.16

mw: 84.40 kDa

protein length: 749 aa

gene length: 2250 bp

function: mitomycin C-specific DNA damage repair

product: helicase

essential: no

synonyms: yprA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,334,581 2,336,830

Phenotypes of a mutant

• sensitive to mitomycin C [pubmed|30379365]

The protein

Protein family

• [SW|helicase family] (according to UniProt)

[SW|Domains]

• [SW|Helicase ATP-binding domain] (aa 63-243) (according to UniProt)
• [SW|Helicase C-terminal domain] (aa 276-430) (according to UniProt)

Structure

• [PDB|2P6R] (the N-terminal domain, corresponds to aa 31 ... 451 of MrfA, 25% identity) [pubmed|17558417]

Expression and Regulation

Operons

• genes: [gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A412 (yprA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/412 NBRP B. subtilis, Japan]
• BKE22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
• BKK22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC

References

Research papers

• 30379365,17558417