SubtiBank SubtiBank
cca
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

cca

tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3 end
locus
BSU22450
pI
8.04
mw
45.29 kDa
protein length
397 aa Sequence Blast
gene length
1191 bp Sequence Blast
function
tRNA modification
product
tRNA nucleotidyltransferase
essential
yes
ec
2.7.7.25
synonyms
papS,ypjI

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,355,880 → 2,357,073

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)
  • Protein family

  • the protein is similar to the ''E. coli'' poly(A) polymerase
  • Structure

  • [PDB|1MIV] (Geobacillus stearothermophilus 44% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
    description
    [Pubmed|20308541]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
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    References

    Reviews

  • 19883645,16364630,20155482,18523015,28117687
  • Original publications

  • 17179213,12526808,20360175,9829937,16109934
  • Operon and expression

  • 20308541,23894131