SubtiBank SubtiBank
pucG
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pucG

(S)-ureidoglycine-glyoxylate aminotransferase
Locus
BSU_32520
Isoelectric point
5.42
Molecular weight
45.58 kDa
Protein length
416 aa Sequence Blast
Gene length
1251 bp Sequence Blast
Function
purine utilization
Product
(S)-ureidoglycine-glyoxylate aminotransferase
Essential
no
Synonyms
yurG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Utilization of nucleotides

    • Gene

    Coordinates
    3,341,166 3,342,416

    The protein

    Catalyzed reaction/ biological activity

  • transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine PubMed
  • (S)-2-ureidoglycine + glyoxylate --> glycine + N-carbamoyl-2-oxoglycine (according to UniProt)

    • Protein family

  • Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

    • Cofactors

  • PLP

    • Structure

  • 3ISL PubMed

    • Expression and Regulation

    Operons

    Genes
    pucF-pucG
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • PucR: activation, PubMed, in PucR regulon

    • Regulation

  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
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    Biological materials

    Mutant

  • MGNA-A942 (yurG::erm), available at the NBRP B. subtilis, Japan
  • BKE32520 (pucG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCCTGACACAGCCATTCCTC, downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC
  • BKK32520 (pucG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCCTGACACAGCCATTCCTC, downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC

    • References

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