tetB

multifunctional tetracycline/Na⁺ resistance protein

locus: BSU_40770

pI: 9.54

mw: 49.59 kDa

protein length: 458 aa

gene length: 1377 bp

function: resistance to tetracyclin

product: multifunctional tetracycline/Na⁺ resistance protein

essential: no

synonyms: tetA,tetA(L)

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,187,681 4,189,057

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)
• [SW|TCR/Tet family] (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|55C9FA5282B213915C164B46119C482D03491381|tetL]-[gene|4B900A64A96696F48BB0C2DC3B0884CD2F5E298B|tetB]
  description: (according to [http://dbtbs.hgc.jp/COG/prom/tetLB.html DBTBS]) null

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2844262], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• GP2080 (''[gene|4B900A64A96696F48BB0C2DC3B0884CD2F5E298B|tetB]''::''cat''), available in [SW|Jörg Stülke]'s lab
• GP2081 (''[gene|4B900A64A96696F48BB0C2DC3B0884CD2F5E298B|tetB]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• BKE40770 ([gene|4B900A64A96696F48BB0C2DC3B0884CD2F5E298B|tetB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATTTCCCCTCCATTAA, downstream forward: _UP4_TAAATGAGTGAAAGGATCAA
• BKK40770 ([gene|4B900A64A96696F48BB0C2DC3B0884CD2F5E298B|tetB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATTTCCCCTCCATTAA, downstream forward: _UP4_TAAATGAGTGAAAGGATCAA

labs

• [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]

References

• 8631673,10735849,8962071,9988470,8849239,2844262,25169108