SubtiBank SubtiBank
nadE
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

nadE

NH3-dependent NAD+ synthetase
locus
BSU03130
pI
4.89
mw
30.24 kDa
protein length
272 aa Sequence Blast
gene length
816 bp Sequence Blast
function
NAD biosynthesis
product
NH3-dependent NAD+ synthetase
essential
yes
ec
6.3.1.5
synonyms
outB, tscBGH, gsp-81

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    338,288 → 339,106

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ (according to Swiss-Prot)
  • Protein family

  • NAD synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|1KQP]
  • Expression and Regulation

    Operons

    genes
    [gene|4B74B1653D783779AFB37EDF9D33E7D6F1AC92B5|nadE]
    description
    [Pubmed|2435704]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9271869,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulation

  • induced by antibiotics interacting with the cytoplasmic membrane [Pubmed|22926563]
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    References

  • 9271869,8450306,19554628,11544224,22926563