SubtiBank SubtiBank
gatC
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

gatC

production of glutamyl-tRNA(Gln)
locus
BSU06670
pI
4.60
mw
10.72 kDa
protein length
gene length
288 bp Sequence Blast
function
translation
product
glutamyl-tRNA(Gln) amidotransferase (subunit C)
essential
yes
ec
6.3.5.7
synonyms
yerL, yedA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • Gene

    Coordinates
    728,732 → 729,022

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP L-glutamyl-tRNA(Gln) L-glutamine = ADP phosphate L-glutaminyl-tRNA(Gln) L-glutamate (according to Swiss-Prot)
  • Protein family

  • gatC family (according to Swiss-Prot)
  • Modification

  • phosphorylated on Arg-3 [Pubmed|22517742]
  • Structure

  • [PDB|2DF4] ([protein|FA88576A405F8C92C83BE74985039DC02E1F6C94|GatA]-[protein|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|GatB]-[protein|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|GatC] complex from ''Staphylococcus aureus'') [Pubmed|16809541]
  • Expression and Regulation

    Operons

    genes
    [gene|4A6AB40A91B4821B99E2FECD1C4E523321B8BDEA|gatC]-[gene|FA88576A405F8C92C83BE74985039DC02E1F6C94|gatA]-[gene|F9C088D2DB3E99FDF961DE1B78A9B81279F204C9|gatB]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A928 (yerL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/928 NBRP B. subtilis, Japan]
  • References

  • 9342321,20882017,22517742,16809541