SubtiBank SubtiBank
ilvH [Fri Apr 01 2016 13:34:43 GMT+0200 (CEST)]
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ilvH [Fri Apr 01 2016 13:34:43 GMT+0200 (CEST)]

acetolactate synthase (small subunit)
locus
BSU28300
pI
10.00
mw
19.00 kDa
function
biosynthesis of branched-chain amino acids
product
acetolactate synthase (small subunit)
essential
no
ec
2.2.1.6
synonyms
ilvN

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CodY regulon], [SW|T-box], [SW|TnrA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,894,733 -> 2,895,251

    The protein

    Catalyzed reaction/ biological activity

  • 2 pyruvate = 2-acetolactate CO2 (according to Swiss-Prot)
  • Protein family

  • acetolactate synthase small subunit family (according to Swiss-Prot)
  • Structure

  • [PDB|2F1F] (regulatory subunit from ''Escherichia coli'', 37% identity, 62% similarity) [Pubmed|16458324]
  • Expression and Regulation

    Operon

  • ''[protein|search|ilvB]-[protein|search|ilvH]-[protein|search|ilvC]-[protein|search|leuA]-[protein|search|leuB]-[protein|search|leuC]-[protein|search|leuD]'' [Pubmed|1577690]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1577690]
  • Regulation

  • for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.]
  • repressed by casamino acids [Pubmed|12107147]
  • expression is stimulated in the presence of glucose [Pubmed|12193635]
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|15547269]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
  • Regulatory mechanism

  • glucose regulation: [protein|search|CcpA] [Pubmed|12193635]
  • repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
  • [protein|search|TnrA]: transcription repression [Pubmed|15547269]
  • [protein|search|CodY]: transcription repression [Pubmed|12618455]
  • References

  • 15060025,12193635,19258532,8289305,18641142,15547269,12618455,15547269,12618455,12107147,20935095,25157083,24163341,25755103,26220295