SubtiBank SubtiBank
mleN [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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mleN [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

malate-H+/Na+-lactate antiporter
locus
BSU23560
pI
7.25
mw
50.00 kDa
function
malate uptake
product
malate-H+/Na+-lactate antiporter
essential
no
synonyms
yqkI

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|utilization of specific carbon sources], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|AnsR regulon], [SW|CcpA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,452,800 -> 2,454,206

    Phenotypes of a mutant

  • abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present [Pubmed|10903309]
  • The protein

    Protein family

  • nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|NhaC]
  • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • Expression and Regulation

    Operon

  • ''[protein|search|ansA]-[protein|search|ansB]-[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
  • ''[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • ''[protein|search|ansA]'': [protein|search|SigA] [Pubmed|1711029]
  • ''[protein|search|mleN]'': [protein|search|SigA] [Pubmed|22900538]
  • Regulation

  • ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
  • ''[protein|search|mleN]'': repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
  • Regulatory mechanism

  • [protein|search|AnsR]: transcription repression [Pubmed|11914346]
  • [protein|search|CcpA]: transcription repression [Pubmed|22900538]
  • Biological materials

    Mutant

  • GP1460 (''mleN''::''spc''), available in [SW|Stülke] lab
  • lacZ fusion

  • pGP388 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References

  • 18763711,10903309,22900538,22383849,1711029,11914346