SubtiBank SubtiBank
ispG
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ispG

similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU25070
pI
6.80
mw
40.42 kDa
protein length
377 aa Sequence Blast
gene length
1131 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
essential
yes
ec
1.17.7.3
synonyms
yqfY

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,589,123 → 2,590,256

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + protein-dithiol (according to Swiss-Prot)
  • Protein family

  • ispG family (according to Swiss-Prot)
  • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • Structure

  • [PDB|3NOY] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|20932974]
  • [SW|Localization]

  • secreted (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|yqfZ]-[gene|4992F5C2ED7DE37C08D5715F7E8851F5FDB51059|ispG]
    description
    [Pubmed|12662922]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]
  • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|12662922,15383836]
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    References

  • 12662922,15383836,17458547,23832336,23840410,20932974