SubtiBank SubtiBank
vpr
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vpr

minor extracellular serine protease
locus
BSU_38090
pI
5.77
mw
85.42 kDa
protein length
806 aa Sequence Blast
gene length
2421 bp Sequence Blast
function
protein degradation
product
minor extracellular serine protease
essential
no
synonyms
ipa-45r

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • Gene

    Coordinates
    3,907,844 3,910,264

    The protein

    Protein family

  • [SW|peptidase S8 family] (according to UniProt)
  • [SW|Domains]

  • Inhibitor I9 domain (aa 57-142) (according to UniProt)
  • Peptidase S8 domain (aa 158-579) (according to UniProt)
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • Expression and Regulation

    Operons

    genes
    [gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|25666135], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|25666135], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16291680], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [Pubmed|26020636], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]
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    Biological materials

    Mutant

  • KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
  • BKE38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT
  • BKK38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT
  • References

    Reviews

  • 20735481
  • Original publications

  • 24115457,16291680,16267290,18957862,25666135,26020636,1938892