SubtiBank SubtiBank
fadN [2019-05-24 09:34:26]
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fadN [2019-05-24 09:34:26]

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
locus
BSU32840
pI
6.53
mw
89.96 kDa
protein length
815 aa Sequence Blast
gene length
2448 bp Sequence Blast
function
fatty acid degradation
product
3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
essential
no
ec
1.1.1.35
synonyms
yusL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

    • Gene

    Coordinates
    3,370,025 3,372,472

    The protein

    Catalyzed reaction/ biological activity

  • (S)-3-hydroxyacyl-CoA NAD = 3-oxoacyl-CoA NADH (according to Swiss-Prot)

    • Protein family

  • 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)

    • [SW|Cofactors]

  • FMN or FAD [Pubmed|21635694]

    • Biological materials

    Mutant

  • MGNA-A604 (yusL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/604 NBRP B. subtilis, Japan]
  • BKE32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
  • BKK32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG

    • References

  • 12817086,12850135,17189250,17919287,23033921,21398533