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mgtE [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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mgtE [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

primary magnesium transporter
locus
BSU13300
pI
4.00
mw
50.00 kDa
function
magnesium uptake
product
primary magnesium transporter
essential
no
synonyms
ykoK

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|M-box]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,396,013 -> 1,397,368

    Phenotypes of a mutant

  • a ''[protein|search|mgtE]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722]
  • a ''[protein|search|mgtE] [protein|search|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722]
  • The protein

    Protein family

  • SLC41A transporter family (according to Swiss-Prot)
  • [SW|Domains]

  • 2 [SW|CBS domain]s (140-203, 204-260)
  • Structure

  • [PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703]
  • [PDB|2QBZ] (the mgtE riboswitch)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Regulation

  • induced at low magnesium concentration ([protein|search|M-box]) [Pubmed|17803910]
  • Regulatory mechanism

  • [protein|search|M-box]: Mg(2)-responsive [SW|riboswitch] [Pubmed|17803910]
  • Biological materials

    Mutant

  • MGNA-B313 (ykoK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]
  • Expression vector

  • pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • References

  • 17803910,15096624,21315082,22970223,24415722,25182490,17700703,25367295