SubtiBank SubtiBank
yisY
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yisY

spore coat protein, similar to chloride peroxidase
Locus
BSU_10900
Isoelectric point
6.89
Molecular weight
30.41 kDa
Protein length
268 aa Sequence Blast
Gene length
807 bp Sequence Blast
Function
protection of the spore
Product
spore coat protein
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class III
  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    1,169,043 1,169,849

    The protein

    Protein family

  • AB hydrolase superfamily (according to UniProt)

    • Domains

  • AB hydrolase-1 domain (aa 23-254) (according to UniProt)

    • Structure

  • 5H3H (from Exiguobacterium Antarcticum, 52% identity)

    • Localization

  • inner spore coat, localization depends on SafA PubMed

    • Expression and Regulation

    Operons

    Genes
    yisY
    Description
    PubMed

    Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon
  • SigK: sigma factor, PubMed, in SigK regulon
  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • GerE: repression, in GerE regulon

    • Regulation

  • expressed during sporulation in the forespore (SigG) PubMed
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    Biological materials

    Mutant

  • MGNA-A501 (yisY::erm), available at the NBRP B. subtilis, Japan
  • BKE10900 (yisY::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCGCATTCCTCCTGTT, downstream forward: _UP4_TAAGAAAAAACCCGACATTG
  • BKK10900 (yisY::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCGCATTCCTCCTGTT, downstream forward: _UP4_TAAGAAAAAACCCGACATTG

    • References

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