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pdhB [Mon Jan 18 2016 15:29:38 GMT+0100 (CET)]
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pdhB [Mon Jan 18 2016 15:29:38 GMT+0100 (CET)]

pyruvate dehydrogenase (E1 beta subunit)
locus
BSU14590
pI
4.00
mw
35.00 kDa
function
links glycolysis and TCA cycle
product
pyruvate dehydrogenase (E1 beta subunit)
essential
no
ec
1.2.4.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|stringent response]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,529,445 -> 1,530,422

    Phenotypes of a mutant

  • defects in sporulation and unable to grow on glucose as single carbon source [Pubmed|11976308]

    • The protein

    Catalyzed reaction/ biological activity

  • Pyruvate [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine CO2 (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|AcoB], [SW|BkdAB]

    • Kinetic information

  • Michaelis-Menten [Pubmed|6414463]

    • Modification

  • phosphorylation on (Ser-302 OR Ser-306) [Pubmed|17218307]

    • Effectors of protein activity

  • Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
  • Low sensibility to NADPH

    • Structure

  • [PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'')

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|PdhA]-[protein|search|PdhB]-[protein|search|PdhC]-[protein|search|PdhD]

    • Expression and Regulation

    Operon

  • ''[protein|search|pdhA]-[protein|search|pdhB]-[protein|search|pdhC]-[protein|search|pdhD]'' [Pubmed|11976308]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|20081037]

    • Regulation

  • expression activated by glucose (2.8-fold) [Pubmed|12850135]
  • subject to negative stringent control upon amino acid limitation [Pubmed|20081037]

    • Regulatory mechanism

  • stringent response: due to presence of guanine at 1 position of the transcript [Pubmed|20081037]

    • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP459 (spc), available in [SW|Stülke] lab

    • lacZ fusion

  • pGP722 (in [protein|search|pAC5]), available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

    • Labs working on this gene/protein

  • [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

    • References

    Reviews

  • 19476487,9655937,2227213,6805383,24798336

    • Original publications

  • 9352926,15378759,12850135,17218307,18763711,6414463,11976308