ytsJ [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH

locus: BSU29220

pI: 5.00

mw: 43.00 kDa

function: malate utilization

product: NADP-dependent malate dehydrogenase

essential: no

ec: 1.1.1.38

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence): 2,989,900 -> 2,991,132

Phenotypes of a mutant

• Poor growth with malate as single carbon source [Pubmed|16788182]

The protein

Catalyzed reaction/ biological activity

• (S)-malate NADP = pyruvate CO₂ NADPH (according to Swiss-Prot) malate <--> pyruvate

Protein family

• malic enzymes family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|MleA]

Structure

• [PDB|1WW8] (from ''Pyrococcus horikoshii'', 46% identity, 63% similarity)

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operon

• ''[protein|search|ytsJ]'' [Pubmed|9387221]

Additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Biological materials

Mutant

• GP612 (spc), available in [SW|Jörg Stülke]'s lab
• GP1143 (spc), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1432 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

FLAG-tag construct

• GP1131 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

• 16479537,16788182,12949160,9387221,23136871,22740702,15378759