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gltA [Mon May 16 2016 16:29:38 GMT+0200 (CEST)]
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gltA [Mon May 16 2016 16:29:38 GMT+0200 (CEST)]

large subunit of glutamate synthase
locus
BSU18450
pI
5.00
mw
168.00 kDa
function
glutamate biosynthesis
product
glutamate synthase (large subunit)
essential
no
ec
1.4.1.13
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|GltC regulon], [SW|FsrA regulon], [SW|TnrA regulon], [SW|Efp-dependent proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,010,070 -> 2,014,632

    Phenotypes of a mutant

  • auxotrophic for glutamate

    • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

    • Protein family

  • glutamate synthase family (according to Swiss-Prot) glutamate synthase family

    • Paralogous protein(s)

  • [protein|search|YerD]

    • [SW|Domains]

  • Glutamine amidotransferase type-2 domain (22-415)
  • Nucleotide binding domain (1060-1112)

    • Modification

  • phosphorylated on Arg-904 AND/OR Arg-914 [Pubmed|22517742]

    • [SW|Cofactors]

  • [3 Fe- 4 S] cluster, FAD, FMN

    • Structure

  • [PDB|2VDC] (the [protein|search|GltA]-[protein|search|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747]

    • [SW|Localization]

  • membrane associated [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 18763711 PubMed], cytoplasm

    • [SW|Interactions]

  • [protein|search|GltA]-[protein|search|GltB] [Pubmed|20933603]
  • [protein|search|GltA]-[protein|search|Icd] [Pubmed|20933603]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]
  • [SW|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [Pubmed|23239624,23239623]

    • Expression and Regulation

    Operon

  • ''[protein|search|gltA]-[protein|search|gltB]'' [Pubmed|12823818]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2548995]

    • Regulation

  • expression activated by glucose (11 fold) ([protein|search|CcpA], [protein|search|GltC]) [Pubmed|12850135,14523131]
  • repressed by arginine ([protein|search|GltC], [protein|search|RocG]) [Pubmed|15150225]
  • expressed in the presence of ammonium [Pubmed|11029411]
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|11029411]
  • part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]

    • Regulatory mechanism

  • [protein|search|GltC]: transcription activation [Pubmed|2548995]
  • [protein|search|TnrA]: transcription repression [Pubmed|11029411]
  • [protein|search|Fur]: indirect effect via control of ''[protein|search|fsrA]'' expression [Pubmed|22389480]
  • [protein|search|FsrA]: translation inhibition [Pubmed|22389480]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • [protein|search|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

    • Biological materials

    Mutant

  • GP807 (del ''[protein|search|gltA]-[protein|search|gltB]''::''tet'') , available in [SW|Jörg Stülke]'s lab
  • GP222 (''gltA'' under the control of p-xyl), available in [SW|Jörg Stülke]'s lab
  • 1A808 ( ''gltA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A808&Search=1A808 BGSC]
  • 1A809 ( ''gltA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A809&Search=1A809 BGSC]

    • lacZ fusion

  • pGP526 (in [protein|search|pAC7]), pGP919 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany
  • [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 16143852,12859215,22625175,12859215

    • Original publications

  • 12823818,18199747,11029411,12850135,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,17981983,18763711,20933603,17012385,22389480,22517742,25755103