gmuA

glucomannan-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIA of the [category|SW 1.2.2|PTS]

locus: BSU_05820

pI: 5.63

mw: 12.21 kDa

protein length: 110 aa

gene length: 333 bp

function: glucomannan uptake and phosphorylation

product: glucomannan-specific lichenan-specific [category|SW 1.2.2|PTS], EIIA component

essential: no

synonyms: ydhN

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 626,933 627,265

The protein

Protein family

• [category|SW 1.2.2|PTS] permease, lactose family [Pubmed|10627040]

Paralogous protein(s)

• [protein|E8B6C8E722BEE866878CDD816B7828C132F94FCD|LicA]

[SW|Domains]

• [SW|PTS EIIA domain] type-3 (aa 9-107) (according to UniProt)

Structure

• [PDB|3K1S] (IIA(Cel) from ''B. anthracis'', 42% identity)

Biological materials

Mutant

• MGNA-C189 (ydhN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2187 NBRP B. subtilis, Japan]
• BKE05820 ([gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGATTCACCATTAA, downstream forward: _UP4_ATATAAAAAATCGGGGTGGA
• BKK05820 ([gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGATTCACCATTAA, downstream forward: _UP4_ATATAAAAAATCGGGGTGGA

References

• 18177310,10627040,18177310,20817675