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zwf
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zwf

glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
locus
BSU_23850
pI
5.28
mw
55.49 kDa
protein length
489 aa Sequence Blast
gene length
1470 bp Sequence Blast
function
initiation of the pentose phosphate pathway
product
glucose 6-phosphate dehydrogenase
essential
no
ec
1.1.1.49
synonyms
yqjJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]

    • Gene

    Coordinates
    2,479,156 2,480,625

    Phenotypes of a mutant

  • suppresses the growth defect of [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutants on gluconeogenic substrates [Pubmed|16272399]

    • The protein

    Catalyzed reaction/ biological activity

  • D-glucose 6-phosphate + NADP+ --> 6-phospho-D-glucono-1,5-lactone + H+ + NADPH (according to UniProt)

    • Protein family

  • glucose-6-phosphate dehydrogenase family (single member, according to UniProt)

    • [SW|Cofactors]

  • Mg2+, Mn2+, Ca2+

    • Effectors of protein activity

  • NAD+, NADP+ and NADPH affect the enzyme kinetic [Pubmed|6292660]

    • Structure

  • [PDB|1DPG] (from ''Leuconostoc Mesenteroides'', 45% identity, 62% similarity) [Pubmed|7881907]

    • Additional information

  • The enzyme is a dimer [Pubmed|6292660]

    • Expression and Regulation

    Operons

    genes
    [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]
    description
    [Pubmed|12850135]

    regulation

  • constitutive [Pubmed|12850135]
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    Biological materials

    Mutant

  • MGNA-C392 (yqjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2390 NBRP B. subtilis, Japan]
  • SM-NB3 (''zwf-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs [pubmed|16272399]
  • BKE23850 ([gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG
  • BKK23850 ([gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAAAAGTACCTCACTTT, downstream forward: _UP4_TAATAAGAAGAAAAAAGCCG

    • FLAG-tag construct

  • GP1401 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

  • 6292660,12850135,19779711,7881907,24571712,16272399