pstBA

phosphate ABC transporter (ATP-binding protein)

Locus: BSU_24960

Isoelectric point: 7.90

Molecular weight: 29.85 kDa

Protein length: 269 aa

Gene length: 810 bp

Function: high-affinity phosphate uptake

Product: phosphate ABC transporter (ATP-binding protein)

Essential: no

E.C.: 3.6.3.27

Synonyms: pstB,yqgJ,yzmE

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of ions
• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,578,003 2,578,812

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + phosphate --> ADP + H⁺ + 2 phosphate (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• GlnQ, ArtR, TcyN, TcyC, YxeO, PstBB

Domains

• ABC transporter domain (aa 23-264) (according to UniProt)

Structure

• 2OLJ (from Geobacillus stearothermophilus, 37% identity)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pstS-pstC-pstA-pstBA-pstBB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PhoP: activation, PhoP-P, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

  Additional information

  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

Mutant

• MGNA-C440 (yqgJ::erm), available at the NBRP B. subtilis, Japan
• BKE24960 (pstBA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC, downstream forward: _UP4_GGGAAATTCGGATAGGAGGG
• BKK24960 (pstBA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC, downstream forward: _UP4_GGGAAATTCGGATAGGAGGG

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

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