paiA

spermine/spermidine-N-acetyltransferase

locus: BSU_32150

pI: 5.02

mw: 19.87 kDa

protein length: 172 aa

gene length: 519 bp

function: control of intracellular polyamine concentrations

product: spermine/spermidine-N-acetyltransferase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.6|Metabolism of polyamines]

Gene

Coordinates: 3,304,743 3,305,261

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + alkane-α,ω-diamine --> N-acetylalkane-α,ω-diamine + CoA + H⁺ (according to UniProt)

[SW|Domains]

• [SW|N-acetyltransferase domain] (aa 3-172) (according to UniProt)

Structure

• [PDB|1TIQ]

Expression and Regulation

Operons

• genes: [gene|430E15D61FCCA20FAC2BCCF504A12070794372FD|paiA]-[gene|511CFC625D79AAA8B78809651684E4E409337AFA|paiB]
  description: [Pubmed|27965289]

Biological materials

Mutant

• BKE32150 ([gene|430E15D61FCCA20FAC2BCCF504A12070794372FD|paiA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCGGATTCAGCCCC, downstream forward: _UP4_TAATATTTTTCGAAGGGTGG
• BKK32150 ([gene|430E15D61FCCA20FAC2BCCF504A12070794372FD|paiA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTCGGATTCAGCCCC, downstream forward: _UP4_TAATATTTTTCGAAGGGTGG

References

• 17588214,16210326,11325965,27965289