SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

ycxA

unknown

locus: BSU_03530

pI: 8.76

mw: 44.70 kDa

protein length: 409 aa

gene length: 1227 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 403,217 → 404,443

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|4264FE54F73766F7029C871E14B47DCFE5E4BC38|ycxA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

  regulation

  • expressed at high cell density ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051]

  additional information

  • [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-C002 (ycxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2000 NBRP B. subtilis, Japan]
• BKE03530 (Δ[gene|4264FE54F73766F7029C871E14B47DCFE5E4BC38|ycxA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGACCTGCCTTTA, downstream forward: _UP4_TCAATATAAAAGGATCAGCA
• BKK03530 (Δ[gene|4264FE54F73766F7029C871E14B47DCFE5E4BC38|ycxA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGACCTGCCTTTA, downstream forward: _UP4_TCAATATAAAAGGATCAGCA

References

• 16091051