SubtiBank SubtiBank
yitJ
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yitJ

bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
locus
BSU_11010
pI
5.34
mw
67.75 kDa
protein length
612 aa Sequence Blast
gene length
1839 bp Sequence Blast
function
methionine biosynthesis, tetrahydrofolate interconversion
product
bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
essential
no
ec
2.1.1.13
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.11|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    1,178,757 1,180,595

    The protein

    Catalyzed reaction/ biological activity

  • S-methyl-L-methionine + L-homocysteine --> 2 L-methionine (according to UniProt)
  • 5-methyltetrahydrofolate + NAD(P)+ --> 5,10-methylenetetrahydrofolate + NAD(P)H (according to UniProt)
  • Protein family

  • C-terminal part: methylenetetrahydrofolate reductase family (single member, according to UniProt)
  • [SW|Domains]

  • Hcy-binding domain (aa 1-280) (according to UniProt)
  • [SW|Cofactors]

  • FAD (according to UniProt)
  • Zn2+ (according to UniProt)
  • Structure

  • [PDB|1Q7M] (the methionine synthase domain, from Thermotoga maritima, 31% identity) [pubmed|14752199]
  • [PDB|6FNU] (the methylene THF reductase domain, from Saccharomyces cerevisiae, 25% identity)
  • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • Expression and Regulation

    Operons

    genes
    [gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]

    description

  • [Pubmed|10094622]
  • regulatory mechanism

  • [regulon|S-box|S-box]: termination/antitermination, [pubmed|10094622], in [regulon|S-box|S-box]
  • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B195 (yitJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1194 NBRP B. subtilis, Japan]
  • BKE11010 ([gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT, downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
  • BKK11010 ([gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT, downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
  • References

    The [protein|search|S-box]

  • 19258532,10094622,18039762,20951706,22543867,29604896,29794222
  • Other publications

  • 19779461,12107147,18763711,22960854,14752199