SubtiBank SubtiBank
topA [Mon Oct 17 2016 15:01:49 GMT+0200 (CEST)]
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topA [Mon Oct 17 2016 15:01:49 GMT+0200 (CEST)]

DNA topoisomerase I
locus
BSU16120
pI
9.00
mw
78.00 kDa
function
DNA replication
product
DNA topoisomerase I
essential
yes
ec
5.99.1.2
synonyms
topI

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA condensation/ segregation], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|Efp-dependent proteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,683,661 -> 1,685,736

    Phenotypes of a mutant

  • essential [Pubmed|25092907]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
  • Protein family

  • prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|TopB]
  • [SW|Domains]

  • N-terminal [SW|TOPRIM domain] (aa 3-114) [Pubmed|9722641]
  • Structure

  • [PDB|2GAI] (from ''Thermotoga maritima '', 42% identity) [Pubmed|16600296]
  • [SW|Interactions]

  • TopA interacts with the [SW|RNA polymerase] [Pubmed|21710567]
  • Expression and Regulation

    Operon

  • ''topA'' [Pubmed|22383849]
  • Additional information

  • [SW|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
  • Biological materials

    Expression vector

  • pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • References

  • 17114254,21710567,25092907,16600296,27378778,9722641