The second international online conference
#Subtillery2021 will be held 14th - 18th June - save the date, for more information see the
conference website!
The 21st
International Conference on Bacilli has been postponed to 2022 and will take place in Prague.
function
relaxation of negatively supercoiled DNA behind RNA polymerase
product
DNA topoisomerase I
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation][category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins][category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]Gene
Coordinates
1,683,661 1,685,736
Phenotypes of a mutant
essential [Pubmed|25092907]non-essential according to [Pubmed|28189581], but only one resistance cassette was possibleessential, deletion of [gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA] results in the immediate acquisition of suppressor mutations that result in overexpression of [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]-[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC] [pubmed|30957856]The protein
Catalyzed reaction/ biological activity
relaxation of negatively supercoiled DNAATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)Protein family
Type IA topoisomerase family (together with [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|TopB])(according to UniProt)Paralogous protein(s)
[protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|TopB] [SW|Domains]
N-terminal [SW|TOPRIM domain] (aa 3-114) [Pubmed|9722641]Structure
[PDB|4RUL] (from ''E. coli'', 40% identity) [Pubmed|26490962]additional information
[protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|TopA] may interact with [SW|RNA polymerase] [pubmed|21710567]mutations affecting Val-44 (V44D) and Ser-478 (S478P) result in TopA hyperactivity [pubmed|29242163]Expression and Regulation
Operons
genes
[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]regulation
repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|12850135]expression is heterogeneous [pubmed|29809139]view in new tabgenes
[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
description
[Pubmed|22383849]
additional information
[SW|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]view in new tabBiological materials
Mutant
BKK16120 (Δ[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCATCCCCCTCAAGA, downstream forward: _UP4_TAGCGGTGAGCAGGGTCTTGGP1963 (Δ[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]::aphA3 (citB-alsT)16) [pubmed|30957856]Expression vectors
pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s labReferences
Reviews
6305585,16936698,29177730 Original publications
17114254,21710567,25092907,16600296,27378778,9722641,28189581,28414321,21710567,29242163,26490962,25232096,23514156,22923519,10713132,12167668,30222737,30957856,30478267,32232337