hfq [Fri Sep 02 2016 13:02:37 GMT+0200 (CEST)]

RNA chaperone

locus: BSU17340

pI: 8.00

mw: 8.00 kDa

function: unknown

product: RNA chaperone

essential: no

synonyms: ymaH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]

[SW|Categories] containing this gene/protein

• [SW|RNA chaperones]

Gene

Coordinates on the chromosome (coding sequence): 1,867,373 -> 1,867,594

The protein

Protein family

• hfq family (according to Swiss-Prot)

Structure

• [PDB|3HSB] (complex with an RNA aptamer) [Pubmed|22053080]

Expression and Regulation

Operon

• ''[protein|search|hfq]'' [Pubmed|23457461]

Regulation

• repressed by glucose (7.7-fold) [Pubmed|12850135]
• expression (mRNA levels) is quite constant during growth in minimal medium [Pubmed|23457461]
• the Hfq protein amount increases upon transition to stationary phase [Pubmed|23457461]

Biological materials

Mutant

• GP22 (cat), available in the [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP460 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1067 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 17395525,15009882,21760622,26773797

Original publications

• 12850135,20445260,23457461,22965117,22053080,24932523,25150227,25915524,27049793