spoVV

transporter for dipicolinic acid across the outer forespore membrane

locus: BSU_15030

pI: 10.27

mw: 44.68 kDa

protein length: 408 aa

gene length: 1227 bp

function: transport of dipicolinic acid across the outer forespore membrane

product: dipicolinic acid transporter

essential: no

synonyms: ylbJ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,570,574 1,571,800

Phenotypes of a mutant

• inactivation of ''[gene|40BCEB3648585961D22B71BBB4FB62EE4B3E360D|spoVV]'' eliminates [SW|sporulation] [Pubmed|26735940], this can be suppressed by inactivation of the genes of the [gene|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[gene|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[gene|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] operon [pubmed|28605069]

The protein

Catalyzed reaction/ biological activity

• transport of dipicolinic acid across the outer forespore membrane [pubmed|28945739]

[SW|Localization]

• outer forespore membrane [pubmed|28945739]

Expression and Regulation

Operons

• genes: [gene|40BCEB3648585961D22B71BBB4FB62EE4B3E360D|spoVV]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922,15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|12662922,15699190]

Biological materials

Mutant

• MGNA-B246 (ylbJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1245 NBRP B. subtilis, Japan]
• BKE15030 ([gene|40BCEB3648585961D22B71BBB4FB62EE4B3E360D|spoVV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTACCTCCCCGATGC, downstream forward: _UP4_ACAAAAAAAGGATGAGTCAA
• BKK15030 ([gene|40BCEB3648585961D22B71BBB4FB62EE4B3E360D|spoVV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTACCTCCCCGATGC, downstream forward: _UP4_ACAAAAAAAGGATGAGTCAA

References

• 12662922,15699190,15383836,26735940,28605069,28945739