mreBH [2018-02-13T02:01:24.000Z]

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for LytE activity

locus: BSU14470

pI: 5.24

mw: 35.53 kDa

protein length: 335 aa

gene length: 1005 bp

function: cell shape determation

product: cell shape-determining protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,516,574 → 1,517,581

The protein

Catalyzed reaction/ biological activity

• forms ring-shaped filaments in a heterologous system [Pubmed|21091501]
• required for the activity of [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE] [Pubmed|23869552,16950129]

Protein family

• ftsA/mreB family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB], [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl]

Structure

• [PDB|1JCF] ([protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] from ''Thermotoga maritima'', 52% identity) [Pubmed|11544518]

[SW|Localization]

• during logarithmic growth, [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|MreBH] forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
• forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
• close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]
• reports on helical structures formed by MreBH [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]
• normal localization depends on the presence of glucolipids, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms irregular clusters in an ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutant [Pubmed|22362028]

Expression and Regulation

Operons

• genes: [gene|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH]-[gene|440E24C24D7B12175A839D7DC310339DF75BCB09|ykpC]
  description: [Pubmed|27965289]

  sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [Pubmed|18156261], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

  regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|24125693], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • expression is increased in an [gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP] mutant [Pubmed|22362028]
  • induced at high temperature ([protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI], [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]) [Pubmed|24125693,18156261]

Biological materials

Mutant

• BKE14470 (Δ[gene|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCACCCAATCTCT, downstream forward: _UP4_TAGTCTTTACCCGCAAAATT
• BKK14470 (Δ[gene|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCACCCAATCTCT, downstream forward: _UP4_TAGTCTTTACCCGCAAAATT

References

Localization

• 17064365,20566861,21636744,21636745,22362028

Other original publications

• 16950129,19643765,19659933,21091501,22069484,23879732,23869552,24125693,11544518,18156261,22362028,27965289