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yonN [2018-02-13T12:09:48.000Z]
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yonN [2018-02-13T12:09:48.000Z]

SPβ prophage-derived DNA-binding protein HU 2
locus
BSU21050
pI
10.23
mw
9.71 kDa
protein length
92 aa Sequence Blast
gene length
276 bp Sequence Blast
function
untwisting of the DNA double helix
product
DNA-binding protein HU 2
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.8|Genetics/ other/ based on similarity]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]

    • Gene

    Coordinates
    2,225,337 → 2,225,615

    The protein

    Catalyzed reaction/ biological activity

  • [protein|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|YonN]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]

    • Protein family

  • glycosyltransferase 1 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs]

    • Structure

  • [PDB|1HUE] ([protein|EFD8FC875B6787B1512C5FC4C81E7E4C9BD93028|Hbs] from B. stearothermophilus, 71% identity) [pubmed|7500343]

    • Biological materials

    Mutant

  • BKE21050 (Δ[gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA, downstream forward: _UP4_TAATCAGTTAATGAGGACGA
  • BKK21050 (Δ[gene|3FFFA28E2C16508C6A94408B739864DB7F8DD1F7|yonN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAATTTCCTCCTAA, downstream forward: _UP4_TAATCAGTTAATGAGGACGA

    • References

  • 21954439,7500343