proJ [Tue Oct 25 2016 13:01:32 GMT+0200 (CEST)]

glutamate 5-kinase

locus: BSU18470

pI: 5.00

mw: 40.00 kDa

function: osmoadaptive de novo production of proline

product: glutamate 5-kinase

essential: no

ec: 2.7.2.11

synonyms: yohA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|coping with hyper-osmotic stress]

Gene

Coordinates on the chromosome (coding sequence): 2,015,733 -> 2,016,848

The protein

Catalyzed reaction/ biological activity

• ATP L-glutamate = ADP L-glutamate 5-phosphate (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|ProB]

Structure

• [PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)

Expression and Regulation

Operon

• ''[protein|search|proH]-[protein|search|proJ]'' [Pubmed|21296969]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|21784929]

Regulation

• expressed under conditions of osmotic stress [Pubmed|21784929]
• induced by cold stress [Pubmed|21296969]

Biological materials

Mutant

• GP817 (spc) available in the [SW|Stülke] lab
• 1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]

References

Reviews

• 25367752

Original publications

• 21296969,25344233,21784929