proJ [2017-8-11 16:0:3]

glutamate 5-kinase

locus: BSU18470

pI: 5.30

mw: 40.20 kDa

protein length: 371 aa

gene length: 1113 bp

function: osmoadaptive de novo production of proline

product: glutamate 5-kinase

essential: no

ec: 2.7.2.11

synonyms: yohA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

Gene

Coordinates: 2,015,733 → 2,016,848

The protein

Catalyzed reaction/ biological activity

• ATP L-glutamate = ADP L-glutamate 5-phosphate (according to Swiss-Prot)

Paralogous protein(s)

• [protein|5592C5163CF024289B850916516E2C8C7AD43136|ProB]

Structure

• [PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)

Expression and Regulation

Operons

• genes: [gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
  description: [Pubmed|21296969]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21784929], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • expressed under conditions of osmotic stress [Pubmed|21784929]

Biological materials

Mutant

• GP817 (spc) available in the [SW|Stülke] lab
• 1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]

References

Reviews

• 25367752

Original publications

• 21296969,25344233,21784929,27766092,28752945