SubtiBank SubtiBank
proJ
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proJ

glutamate 5-kinase
locus
BSU_18470
pI
5.30
mw
40.20 kDa
protein length
371 aa Sequence Blast
gene length
1116 bp Sequence Blast
function
osmoadaptive de novo production of proline
product
glutamate 5-kinase
essential
no
ec
2.7.2.11
synonyms
yohA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • Gene

    Coordinates
    2,015,733 2,016,848

    The protein

    Catalyzed reaction/ biological activity

  • ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)
  • Protein family

  • glutamate 5-kinase family (with [protein|5592C5163CF024289B850916516E2C8C7AD43136|ProB], according to UniProt)
  • Paralogous protein(s)

  • [protein|5592C5163CF024289B850916516E2C8C7AD43136|ProB]
  • Structure

  • [PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
    description
    [Pubmed|21296969]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21784929], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulation

  • expressed under conditions of osmotic stress [Pubmed|21784929]
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    Biological materials

    Mutant

  • GP817 (spc) available in [SW|Jörg Stülke]'s lab
  • 1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]
  • BKE18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
  • BKK18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG, downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
  • References

    Reviews

  • 25367752
  • Original publications

  • 21296969,25344233,21784929,27766092,28752945