SubtiBank SubtiBank
rnjA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

rnjA

RNase J1, important for the degradation of RNA fragments containing transcription terminators
locus
BSU14530
pI
5.90
mw
61.34 kDa
protein length
555 aa Sequence Blast
gene length
1665 bp Sequence Blast
function
RNA processing
product
RNase J1
essential
no
synonyms
ykqC

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,523,118 → 1,524,785

    Phenotypes of a mutant

  • growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) [pubmed|26883633]
  • the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]
  • it is not possible to construct a ''[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA] [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny]'' double mutant [Pubmed|23504012]
  • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades [protein|8950A485A0FEA4BA2FFC0C74A8D3D5D73D44B3FF|SR5] and ''[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]'' RNAs [pubmed|27449348]
  • degrades [gene|search|yflS ]mRNA [pubmed|28436820]
  • Protein family

  • RNase J subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|B73CE26E7AEFACFA82C67F3E7DBBB3F766F739CA|RnjB]
  • Effectors of protein activity

  • initeraction with [protein|EB6512177418B1601C6641FB2DEE99C2CD10E671|GapA]-[protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|Sr1] stimulates activity [Pubmed|27449348]
  • Structure

  • [PDB|3ZQ4] [Pubmed|21893285]
  • [SW|Localization]

  • cytoplasm, colocalizes with the ribosomes [Pubmed|27708634]
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • required for [gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS] RNA processing, involved in maturation of the 5’-end of the 16S rRNA
  • Expression and Regulation

    Operons

    genes
    [gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]-[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]
    description
    [Pubmed|24187087]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|24187087], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
  • SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab
  • GP2506 (Δ[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]::''spc''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab
  • pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • pGP418 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1694 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjA-sfGFP''::''spc'' under a xylose-inducible promoter in '' B. subtilis'', available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]
  • GP1722 (in [SW|pBP43]), expression of '' rnjA-GFP''::''spc'' under the native promoter, available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
  • FLAG-tag construct

  • GP1020 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • GP1075 (aphA3), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
  • [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
  • [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
  • References

    Reviews

  • 20458164,21334965,21893280,23403287,21957024,22568516,24064983,25292357,27750368,29314657
  • Original publications

  • 25940620,18079181,19553197,17981983,19458035,15831787,18204464,18713320,19193632,17005971,18445592,17512403,17229210,17576666,19210617,19633085,19638340,19850915,19880604,20025672,20418391,20572937,21803996,21862575,21925382,22198292,22412379,23504012,21893285,21893286,21908660,24187087,26253740,26802042,26940229,27708634,27449348,28277929,28436820,28542621,28795788,26883633