SubtiBank SubtiBank
gerD
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gerD

scaffold of the germinosome, required for clustering of germinant receptors in the spore inner membrane
locus
BSU01550
pI
4.86
mw
20.97 kDa
protein length
185 aa Sequence Blast
gene length
558 bp Sequence Blast
function
germination
product
lipoprotein
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    158,515 159,072

    Phenotypes of a mutant

  • defective in [SW|germination] in response to nutrients [Pubmed|24530795]
  • The protein

    Catalyzed reaction/ biological activity

  • required for clustering of germinant receptors in the spore inner membrane [Pubmed|21696470]
  • [SW|Domains]

  • has an N-terminal signal sequence and a signal for fixation to lipid [Pubmed|23335419]
  • Structure

  • [PDB|4O8W] [Pubmed|24530795]
  • [SW|Localization]

  • outer surface of the inner spore membrane [Pubmed|23335419,21696470]
  • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction [Pubmed|19332816]
  • Additional information

  • 3,500 molecules are present per spore [Pubmed|23749970]
  • Expression and Regulation

    Operons

    genes
    [gene|3E5B9AC0C6F974800DE1ABF25769D24947772DCB|gerD]
    description
    [Pubmed|2517635]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1906867,15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • regulation

  • expression level depends on sporulation conditions (medim composition, temperature) [Pubmed|22327596]
  • additional information

  • 3,500 molecules are present per spore [PubMed|23749970]
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    Biological materials

    Mutant

  • 1A677 ( ''gerD''::''erm''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A677&Search=1A677 BGSC]
  • 1G17 ( ''gerD''::''spec''), [Pubmed|15126458], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1G17&Search=1G17 BGSC]
  • BKE01550 ([gene|3E5B9AC0C6F974800DE1ABF25769D24947772DCB|gerD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTAAGCTCCTTTCAC, downstream forward: _UP4_TAAAGGGAAAGCCGGGATCT
  • BKK01550 ([gene|3E5B9AC0C6F974800DE1ABF25769D24947772DCB|gerD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTAAGCTCCTTTCAC, downstream forward: _UP4_TAAAGGGAAAGCCGGGATCT
  • Antibody

  • available in [SW|Anne Moir] lab
  • labs

  • [SW|Anne Moir], University of Sheffield, UK
  • References

  • 1906867,19332816,17122337,21725007,18556788,19332816,2517635,21696470,22327596,22493018,23335419,23749970,21398556,24530795,24752279,26731423