yhaO

similar to DNA double-strand break repair protein

locus: BSU_09910

pI: 5.23

mw: 46.65 kDa

protein length: 408 aa

gene length: 1227 bp

function: unknown

product: unknown

essential: no

synonyms: sbcF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]

Gene

Coordinates: 1,064,846 1,066,072

The protein

Protein family

• [SW|Metallophosphoesterase superfamily] (according to UniProt)

Structure

• [PDB|4TUG] (from Methanocaldococcus jannaschii, corresponds to aa 5 - 290, 27% identity) [pubmed|25107472]

[SW|Localization]

• cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|3E27C881BB3DE3FD55B7AD21B8771A859D80786F|yhaO]-[gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]-[gene|6D585EB5E56A1E5C1C31680D960043BA08C817E2|yhaM]
  description: [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]

Biological materials

Mutant

• MGNA-A664 (yhaO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/664 NBRP B. subtilis, Japan]
• BKE09910 ([gene|3E27C881BB3DE3FD55B7AD21B8771A859D80786F|yhaO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGCTCCTTTCCAGGA, downstream forward: _UP4_CTTAAGGTGCTTGATACATG
• BKK09910 ([gene|3E27C881BB3DE3FD55B7AD21B8771A859D80786F|yhaO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGCTCCTTTCCAGGA, downstream forward: _UP4_CTTAAGGTGCTTGATACATG

References

• 16267290,16479537,25107472