sigI

[SW|RNA polymerase ][SW|sigma factor ]SigI

locus: BSU_13450

pI: 8.31

mw: 29.04 kDa

protein length: 251 aa

gene length: 756 bp

function: control of a class of heat shock genes

product: [SW|RNA polymerase ][SW|sigma factor ]SigI

essential: no

synonyms: ykoZ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates: 1,411,892 1,412,647

Phenotypes of a mutant

• temperature-sensitive growth on agar plates [Pubmed|11157964]

The protein

Protein family

• [SW|Sigma-70 factor family] (according to UniProt)

Structure

• [PDB|6IVU] (from Hungateiclostridium thermocellum, corresponds to aa 141 ... 245, 38% identity) [pubmed|31106374]

[SW|Localization]

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000194 PRODORIC2 database]

Additional information

• overexpression of SigI suppresses ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [gene|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH] [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]'' mutants [Pubmed|19114499]

Expression and Regulation

Operons

• genes: [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]-[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]
  description: [Pubmed|17185538]

  sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [Pubmed|17185538], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|23199363], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|24125693,23199363], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • induced at high temperature ([protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI], [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]) [Pubmed|23199363,17185538]

Biological materials

Mutant

• MGNA-A229 (ykoZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/229 NBRP B. subtilis, Japan]
• BKE13450 ([gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCAGTTCCTCCCTATA, downstream forward: _UP4_TATCTTAAAGGGGTGCTGCA
• BKK13450 ([gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCAGTTCCTCCCTATA, downstream forward: _UP4_TATCTTAAAGGGGTGCTGCA

References

• 19114499,17185538,11157964,18156261,21541672,24125693,23199363,26744224,28333276,29914988,31106374