fatR

transcriptional repressor of the fatR-yrhJ operon

Locus: BSU_27170

Isoelectric point: 5.62

Molecular weight: 22.07 kDa

Protein length: 194 aa

Gene length: 585 bp

Function: unknown

Product: transcriptional repressor of the fatR-yrhJ operon (TetR family)

Essential: no

Synonyms: yrhI,bscR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Electron transport/ other
• Metabolism → Lipid metabolism → Lipid metabolism/ other
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Gene

Coordinates: 2,777,070 2,777,654

The protein

Protein family

• TetR family

Domains

• HTH tetR-type domain (aa 6-66) (according to UniProt)

Modification

• phosphorylated by PtkA on Tyr-45, this results in displacement of FatR from the DNA PubMed

Structure

• 4ME9 (from B. cereus, 38% identity)

Expression and Regulation

Operons

• Genes: fatR-yrhJ
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigW: sigma factor, PubMed, in SigW regulon
  • SigX: sigma factor, PubMed, in SigX regulon

  Regulatory mechanism

  • FatR: repression, PubMed, in FatR regulon

  Regulation

  • expression is reduced in a SigV mutant PubMed

  Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed

• Genes: yrhH-fatR-yrhJ
  Description: PubMed

  Regulation

  • Expressed under stress conditions (SigW, SigX) PubMed

  Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed

Biological materials

Mutant

• MGNA-A177 (yrhI::erm), available at the NBRP B. subtilis, Japan
• BKE27170 (fatR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTAATCTGACAACCTCC, downstream forward: _UP4_CAAAAATAGGAAAGGGAGAT
• BKK27170 (fatR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTAATCTGACAACCTCC, downstream forward: _UP4_CAAAAATAGGAAAGGGAGAT

References

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