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polA [2017-8-11 16:0:3]
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polA [2017-8-11 16:0:3]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage
locus
BSU29090
pI
4.92
mw
98.89 kDa
protein length
880 aa Sequence Blast
gene length
2640 bp Sequence Blast
function
DNA replication
product
DNA polymerase I
essential
no
ec
2.7.7.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • Gene

    Coordinates
    2,973,182 → 2,975,824

    Phenotypes of a mutant

  • strongly reduced stationary phase mutagenesis [Pubmed|27399782]
  • The protein

    Catalyzed reaction/ biological activity

  • has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
  • Protein family

  • DNA polymerase type-A family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|ExoR], one of the two proteins has to be present for viability [Pubmed|17114254]
  • Structure

  • [PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
  • Expression and Regulation

    Operons

    genes
    [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
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    References

  • 17114254,16045613,17905985,27399782