SubtiBank SubtiBank
murC
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

murC

UDP-N-acetylmuramoyl-L-alanine synthetase
locus
BSU29790
pI
5.31
mw
48.20 kDa
protein length
432 aa Sequence Blast
gene length
1296 bp Sequence Blast
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylmuramoyl-L-alanine synthetase
essential
yes
ec
6.3.2.8
synonyms
ytxF

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,048,177 → 3,049,475

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • murC is dispensable in wall-less L-forms [Pubmed|24704074]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP UDP-N-acetylmuramate L-alanine = ADP phosphate UDP-N-acetylmuramoyl-L-alanine (according to Swiss-Prot)
  • Protein family

  • murCDEF family (according to Swiss-Prot)
  • Structure

  • [PDB|1GQQ] (from Haemophilus influenzae, 31% identity)
  • [SW|Localization]

  • cytoplasm (Homogeneous) [Pubmed|16479537]
  • forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [Pubmed|20233306]
  • Expression and Regulation

    Operons

    genes
    [gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]
    description
    [Pubmed|8733232]
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    References

  • 18063720,8733232,24704074,16479537,20233306