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pxpC
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pxpC

subunit of ATP-dependent 5-oxoprolinase, antagonist of [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|PxpB], control of the [SW|phosphorelay]
locus
BSU_04090
pI
5.47
mw
36.40 kDa
protein length
335 aa Sequence Blast
gene length
1008 bp Sequence Blast
function
detoxification of 5-oxoproline, control of the [SW|phosphorelay], initiation of [SW|sporulation]
product
subunit of 5-oxoprolinase, antagonist of [SW|KipI]
essential
no
synonyms
kipA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]

    • Gene

    Coordinates
    460,592 461,599

    Phenotypes of a mutant

  • no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]
  • reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]

    • The protein

    Catalyzed reaction/ biological activity

  • 5-oxoproline + ATP --> glutamate + ADP + Pi [pubmed|28830929]

    • Protein family

  • PxpC family (single member, according to UniProt)

    • Kinetic information

  • Km (5-oxoproline): 39 M [pubmed|28830929]

    • Structure

  • [PDB|5DUD] ( the YbgJ-YbgK complex from E. coli, 36% identity)

    • Biological materials

    Mutant

  • BKE04090 ([gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG
  • BKK04090 ([gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG

    • References

  • 9334321,21050859,25755103,28830929