nin

inhibitor of the DNA degrading activity of [protein|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|NucA]

locus: BSU_03420

pI: 4.92

mw: 14.86 kDa

protein length: 132 aa

gene length: 399 bp

function: genetic transformation, DNA uptake

product: inhibitor of the DNA degrading activity of [protein|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|NucA]

essential: no

synonyms: comJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 371,729 372,127

The protein

Modification

• phosphorylated on Arg-96 [Pubmed|22517742]

Structure

• [PDB|4MQD]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]-[gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]
  description: [Pubmed|11359569]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|7746143], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

Biological materials

Mutant

• BKE03420 ([gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGCTGTTCCTCCTCT, downstream forward: _UP4_TAATGAAAAACCCCTGAGAG
• BKK03420 ([gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGCTGTTCCTCCTCT, downstream forward: _UP4_TAATGAAAAACCCCTGAGAG

References

• 11814663,11359569,7746143,22517742