ahpT

thiol disulfide oxidoreductase, reduces [protein|B283B702D917E310130BC33A40BF6A5853C3D4C1|AhpA]

locus: BSU_14230

pI: 6.33

mw: 17.66 kDa

protein length: 148 aa

gene length: 447 bp

function: protection of proteins against oxidative damage

product: thiol disulfide oxidoreductase

essential: no

synonyms: ykuV

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 1,492,875 1,493,321

The protein

[SW|Domains]

• [SW|Thioredoxin domain] (aa 2-145) (according to UniProt)

Structure

• [PDB|2B5X] (reduced form), [PDB|2B5Y] (oxidized form)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B283B702D917E310130BC33A40BF6A5853C3D4C1|ahpA]-[gene|398E6FAC317D9BD81FB80A7CC9DF1CC757F2FA98|ahpT]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• GP1729 [gene|search|ahpAT]::kan trpC2 available in [SW|Jörg Stülke]'s lab
• MGNA-B344 (ykuV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1343 NBRP B. subtilis, Japan]
• BKE14230 ([gene|398E6FAC317D9BD81FB80A7CC9DF1CC757F2FA98|ahpT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCCTCCTAT, downstream forward: _UP4_TAGGTATCTGACTAAATAGT
• BKK14230 ([gene|398E6FAC317D9BD81FB80A7CC9DF1CC757F2FA98|ahpT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCCTCCTAT, downstream forward: _UP4_TAGGTATCTGACTAAATAGT

References

• 20817675,16418167,26787766