spsC

aminotransferase, legionaminic acid synthesis

locus: BSU_37890

pI: 5.55

mw: 43.03 kDa

protein length: 389 aa

gene length: 1170 bp

function: legionaminic acid synthesis

product: aminotransferase

essential: no

synonyms: ipa-65d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.8|Biosynthesis of legionaminic acid (for spore crust))]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,889,732 3,890,901

Phenotypes of a mutant

• less hydrophobic spore surface [pubmed|32817102]

The protein

Catalyzed reaction/ biological activity

• legionaminic acid synthesis [pubmed|32817102]

Protein family

• DegT/DnrJ/EryC1 family (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|EpsN] and [protein|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|NtdA], according to UniProt)

[SW|Cofactors]

• PLP [pubmed|12429098]

Structure

• [PDB|1MDX] (ArnB from Salomonella typhimurium, 44% identity) [pubmed|12429098]

[SW|Localization]

• binds the outer surface of the forming spore [Pubmed|14602648]

Expression and Regulation

Operons

• genes: [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• CS218 (''[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE37890 ([gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA
• BKK37890 ([gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA

References

• 9353933,15383836,14602648,25239894,26577401,27197833,12429098,32817102