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exoA [2018-07-30 12:42:23]
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exoA [2018-07-30 12:42:23]

apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance
locus
BSU40880
pI
4.59
mw
29.10 kDa
protein length
252 aa Sequence Blast
gene length
756 bp Sequence Blast
function
repair of oxidative DNA damage in spores
product
apurinic/apyrimidinic endonuclease
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    4,197,780 → 4,198,538

    Phenotypes of a mutant

  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]

    • The protein

    Catalyzed reaction/ biological activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)

    • Protein family

  • DNA repair enzymes AP/exoA family (according to Swiss-Prot)

    • Structure

  • [PDB|5CFE] [Pubmed|27343627]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|38CC84343C47CBF37F007E9056A9248869C396E1|exoA]-[gene|C14B113B1A8BEA0547459AA7EC5755634D1A8A88|ccpB]
    description
    (according to [http://dbtbs.hgc.jp/COG/prom/exoA-ccpA.html DBTBS]) null

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16237020], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16237020]
    • view in new tab

    Biological materials

    Mutant

  • GP898 (Δ''exoA''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP1503 (Δ''exoA''::''kan'', Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''cat''), available in [SW|Jörg Stülke]'s lab
  • BKE40880 (Δ[gene|38CC84343C47CBF37F007E9056A9248869C396E1|exoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCATCCTCCCCCCT, downstream forward: _UP4_TGATGAGATAGCAGTAAGGA
  • BKK40880 (Δ[gene|38CC84343C47CBF37F007E9056A9248869C396E1|exoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCATCCTCCCCCCT, downstream forward: _UP4_TGATGAGATAGCAGTAAGGA

    • References

    Reviews

  • 22933559

    • Original publications

  • 24244006,18203828,16237020,19930460,10540738,21441501,24123749,24123749,24914186,27343627