SubtiBank SubtiBank
oppF
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oppF

oligopeptide [SW|ABC transporter] (ATP-binding protein)
locus
BSU11470
pI
6.95
mw
34.59 kDa
protein length
305 aa Sequence Blast
gene length
918 bp Sequence Blast
function
initiation of sporulation, competence development
product
oligopeptide [SW|ABC transporter] (ATP-binding protein)
essential
no
synonyms
spo0KE

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.3|Uptake of peptides]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    1,224,533 1,225,450

    The protein

    Catalyzed reaction/ biological activity

  • required for the uptake of quorum sensing peptides such as [protein|4FA7D6E6316A6FC71C10C692D125833263894020|PhrH] [Pubmed|21908671]
  • Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)
  • Paralogous protein(s)

  • [protein|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|AppF], [protein|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|YkfD]
  • Structure

  • [PDB|3DHW] (MetN from E. coli, 33% identity) [pubmed|18621668]
  • [SW|Localization]

  • attached to the cell membrane (via [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|OppB]-[protein|DDBCE73B6AF39E5D669475479186C93F287DA9FC|OppC]) [Pubmed|10092453]
  • Expression and Regulation

    Operons

    genes
    [gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]
    description
    [Pubmed|1901616]

    regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, no derepression occurs, however, in a [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY] mutant, due to increased repression by [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC] [Pubmed|25966844], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|10383984], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
  • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|trpS]' and '[protein|search|oppA]' [PubMed|20525796]
  • view in new tab

    Biological materials

    Mutant

  • GP2097 (D(''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE11470 ([gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTCAACTTCTCTC, downstream forward: _UP4_TGATTCATCAATCCTTCAAG
  • BKK11470 ([gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTCAACTTCTCTC, downstream forward: _UP4_TGATTCATCAATCCTTCAAG
  • References

  • 27231947,1901616,10092453,9252573,1899858,21908671,12823818,10383984,25966844,25755103,18621668