SubtiBank SubtiBank
prs
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prs

phosphoribosylpyrophosphate synthetase, universally conserved protein
locus
BSU00510
pI
5.89
mw
34.71 kDa
protein length
317 aa Sequence Blast
gene length
951 bp Sequence Blast
function
phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
product
phosphoribosylpyrophosphate synthetase
essential
yes
ec
2.7.6.1
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    57,745 → 58,698

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family

  • ribose-phosphate pyrophosphokinase family (according to Swiss-Prot)
  • Effectors of protein activity

  • subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]
  • Structure

  • [PDB|1IBS] [PDB|1DKR][PDB|1DKU]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • [SW|universally conserved protein]
  • Expression and Regulation

    Operons

    genes
    [gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|8522540], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
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    Biological materials

    Expression vector

  • N-terminal Strep-tag, expression in ''E. coli'', in [SW|pGP172]: pGP2541, available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 28031352
  • Original publications

  • 2169413,8522540,12837783,12837784,19446032,22083279