SubtiBank SubtiBank
sigA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

sigA

[SW|RNA polymerase] major [SW|sigma factor] SigA
locus
BSU25200
pI
4.63
mw
42.80 kDa
protein length
371 aa Sequence Blast
gene length
1113 bp Sequence Blast
function
transcription
product
[SW|RNA polymerase] major [SW|sigma factor] SigA
essential
yes
synonyms
rpoD,crsA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,600,214 → 2,601,329

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Protein family

  • sigma-70 factor family (according to Swiss-Prot)
  • Structure

  • [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 69% identity) [Pubmed|19903881]
  • [SW|Localization]

  • cytoplasm [Pubmed|25313396]
  • Expression and Regulation

    Operons

    genes
    [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
    description
    [Pubmed|3919021]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|3919021], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]
  • view in new tab

    genes
    [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
    description
    [Pubmed|3298998]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response
  • additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]
  • view in new tab

    Labs working on this gene/protein

  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
  • References

    Reviews

  • 22210308,24308327
  • Original publications

  • 12486038,10556308,8107087,14762002,7918363,8071196,7630711,9185571,9287022,14679239,14976210,19735077,2997585,9658000,3127379,10438769,11535782,7599136,9144176,19680289,20525796,22333917,20935043,20817769,23875654,25112476,26582911,19903881,27977677,28539362,28648455