SubtiBank SubtiBank
bglP
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

bglP

[SW|trigger enzyme],beta-glucoside uptake and control of [protein|search|LicT ]activity
locus
BSU39270
pI
6.81
mw
64.52 kDa
protein length
609 aa Sequence Blast
gene length
1827 bp Sequence Blast
function
beta-glucoside uptake and phosphorylation, control of [protein|search|LicT ]activity
product
[SW|trigger enzyme]
essential
no
ec
2.7.1.69
synonyms
sytA

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
  • Gene

    Coordinates
    4,033,778 → 4,035,607

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family

  • PTS permease, sucrose permease (Scr) family [Pubmed|10627040]
  • [SW|Localization]

  • cell membrane [Pubmed|23475962]
  • Expression and Regulation

    Operons

    genes
    [gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-[gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]-[gene|5A4CDE1CFE319E4471A15702ED1632C96270528D|yxiE]
    description
    [Pubmed|7883710]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7559347], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] binding site overlaps -35 region) [Pubmed|7559347], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]: anti-termination, via [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent [SW|RNA switch], lack of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent antitermination in the presence of gucose due to the requirement of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT] to be phosphorylated by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK], in [regulon|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT regulon]
  • regulation

  • induced by salicin ([protein|search|LicT]) [Pubmed|7883710]
  • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|bglP]' [PubMed|20525796]
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    Biological materials

    Mutant

  • GP475 (erm), available in the [SW|Stülke] lab
  • BKE39270 (Δ[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
  • BKK39270 (Δ[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA
  • Expression vector

  • pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Stülke] lab,
  • pGP1300 (expression of ''[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Stülke] lab
  • lacZ fusion

  • pGP600 (in [protein|search|pAC6]), available in [SW|Stülke] lab
  • References

  • 16672620,20525796,10627040,7883710,8626332,7559347,22900538,23475962